技术专题
喜报，if=9.56，西南大学科研团队揭示蚜虫翅型分化的分子调控机制
文章来源：genecreate
作者：genecreate
发布时间：2020-04-23 14:10
喜报
if=9.56，西南大学植物保护学院王进军教授团队近日在《美国科学院院刊》（pnas）上在线发表题为“the mir-9b microrna mediates dimorphism and development of wing in aphids”的研究论文，发现小分子rna介导生物胁迫因子调控蚜虫翅型分化与翅发育的分子机制，研究结果有利于寻获新的小分子rna控制剂靶标，为蚜虫类害虫防控提供新的思路。（金开瑞合作技术：ip级多克隆抗体制备）
论文链接
（信息来源：西南大学植物保护学院和pnas杂志威尼斯2299官网）
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合作技术：抗体制备
抗体：zmrem1.3兔多抗
western blot analysis of zmrem1.3 in transgenic maize lines 227, 229, 231 and 233 as well as the corresponding non-transgenic sib lines, with b-actin as the loading control.
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合作技术：抗体制备
抗体：多种兔多抗和鼠单抗
polyclonal rabbit antibodies against 60s ribosomal proteins rpl28, rpl26, and 40s ribosomal protein s4 (rps4), and the monoclonal mouse antibody against major royal jelly protein 1 (mrjp1) were developed (genecreate biological engineering, wuhan, china).
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合作技术：抗体制备
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合作技术：抗体制备
抗体：抗crd 1多克隆抗体
(c) immunostaining of crd1-gfp (red fluorescence) in cross-sections of root tips of hj2 (upper panel) and crd1-gfp lines (lower panel). (d) validation of crd1 antibody (anti-crd1) using immunoblot analysis. (e) subcellular localization of crd1 in hj2 analyzed by immunoblot analysis.
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multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array，analytica chimica acta.
合作技术：抗体制备
抗体：chil-4和chifn-4的单抗、纯化的重组chil-4和chifn-1抗原
for the first incubation, the surface capture of the cytokines by their respective primary antibodies anti-chil-4 and anti-chifn-γ, the subsequent cl intensity value increased with increasing incubation time up to a maximum at 30 min (fig.2a). for the second step, formation of the sandwich via the attachment of the ab2-aunp-hrp probe to the exposed ab1-cytokine-immunocomplex, the maximum cl value was similarly reached with only a 25 min incubation (fig.2b).
